Fig. 1.

MET-induced increase of RELN and GAD₆₇ but not GAD₆₅ promoter methylation in mouse FC. MET (filled bars) (5.2 mmol/kg s.c. twice a day) or vehicle (VEH) (open bars) was administered to mice for 6 days. MSP was used to evaluate the percentage of methylated or unmethylated RELN, GAD₆₇, or GAD₆₅ promoter CpG sites (A₃₀₂ ratio of methylated DNA/unmethylated DNA). At the bottom is a representative agarose gel electrophoresis showing increased RELN and GAD₆₇ methylated promoters (M) and decreased unmethylated promoters (U) in MET-treated mice. The data represent the mean ± SE of three to five mice. ∗, P < 0.01 vs. MET-treated group (Student's t test). The last injection of MET was administered 2 h before decapitation.