Fig. 5.

Reversal of MET-induced reelin and GAD₆₇ expression down-regulation after MET withdrawal. (A) Average reelin, GAD₆₇, and GAD₆₅ protein levels (OD ratios of reelin, GAD₆₇, and GAD₆₅ to β-actin) in FC of mice treated with MET (5.2 mmol/kg s.c. twice a day) for 7 days (MET-7d), shaded bars; VEH, open bars; or after 7 days of MET withdrawal (MET-W), filled bars. ∗, P < 0.05 vs. VEH group (Student's t test). (B) Typical Western blots of reelin and GAD₆₇/GAD₆₅ with three serial dilutions of the same sample. The intensity of β-actin determined on the same blot was used for a comparative estimation of sampling variation. The data represent the mean ± SE of three to five mice.