. 2003 Aug;14(8):3114–3125. doi: 10.1091/mbc.E02-12-0804

Table 2.

Plasmids used in this study

Name	Gene Expressed	Vector	Insertion sites	Source
pADH-mycSSO1^a	SSO1	pAD6	SalI and SacI	J. Gerst
pADH-mycNTSSO1	SSO1^2-146	pAD6	SalI and SacI	This study
pADH-mycSSO1Δ1-103^a	SSO1^Δ1-103	pAD6	SalI and SacI	This study
pADH-mycSSO1Δ1-146^a	SSO1^Δ1-146	pAD6	SalI and SacI	This study
pADH-mycSSO1A66^a ^c	SSO1^A66	pAD6	SalI and SacI	J. Gerst
pADH-mycSSO1A79^a ^c	SSO1^A79	pAD6	SalI and SacI	J. Gerst
pADH-mycSSO1D79^c	SSO1^D79	pAD6	SalI and SacI	This study
pGexSSO11-265^d	SSO1^1-265	pGex-4T-3		P. Brennwald
pGexSSO11-265,A66^a ^c	SSO1^1-265,A66	pGex-4T-3	EcoRI and XhoI	This study
pGexSSO11-265,A79^a ^c	SSO1^1-265,A79	pGex-4T-3	EcoRI and XhoI	This study
pGexSSO11-265,D79^c	SSO1^1-265,D79	pGex-4T-3	EcoRI and XhoI	This study
pMM251	SSO1			M. Munson
pMM255	SSO1^E84,E95,A148			M. Munson
pMM256	SSO1^{A95,A99,A119,A123,A148}			M. Munson
pMM255^A66	SSO1^{E84,A66,E95,A148}			This study
pMM255^A79	SSO1^{E84,A79,E95,A148}			This study
pHIS₆-Vsm1^b	VSM1	pTrc-HisB	XhoI and KpnI	J. Gerst
pHIS₆-HAVsm1	HAVSM1	pTrc-HisB	XhoI and KpnI	This study
pADHU-HAVsm1	HAVSM1	pUV2	BamHI	J. Gerst
pADH-myc-Vsm1^2-402	VSM1^2-402	pAD6	SalI and SacI	This study
pGex-TPK1^a	TPK1	pGex-4T-3	EcoRI and XhoI	J. Gerst
pGex-Sec9402-651^d	SEC9^402-651	pGex-4T-3		P. Brennwald
pHIS₆-Snc1^2-94	SNC1^2-94	pTrc-HisA	SacI and HindIII	J. Gerst
pHIS₆-Snc2^2-93	SNC2^2-93	pTrc-HisA	SacI and HindIII	J. Gerst

Plasmid described in Marash and Gerst (2001).

Plasmid described in Lustgarten and Gerst (1999).

Mutant Sso genes were constructed by site-directed mutagenesis. As templates for mutagenesis, pAD6-SSO1 and pGEX-SSO1^1-265 were used, where appropriate.

Plasmid described in Rossi et al. (1997).