Figure 2.

GAL1-TEL1 induction triggers telomere lengthening and checkpoint activation in cells with short telomeres. Wild-type (K699), TEL1 GAL1-TEL1 (YLL1133), TEL1 GAL1-TEL1 yku70Δ (YLL1230), and yku70Δ (YLL941) cell cultures exponentially growing in YEP + raf for >80 generations (time 0) were shifted to YEP + raf + gal and samples were taken at the indicated times to analyze DNA content by FACS analysis (A), telomere length (B, top), and the pattern of Rad53 phosphorylation by Western blot by using anti-Rad53 antibodies (B, bottom). Genomic DNA in B was digested with XhoI and hybridized with a poly(GT) telomere-specific probe.