Figure 7.

Kinetics of telomere elongation of galactose-induced GAL1-TEL1 cells in the presence of high copy number SAE2 and RIF2. GAL1-TEL1 [2 μ vector], GAL1-TEL1 [2 μ SAE2], and GAL1-TEL1 [2 μ RIF2] cell cultures exponentially growing in SC-ura + raf for >80 generations (time 0) were shifted to SC-ura + raf + gal and samples were taken at the indicated times to analyze telomere length (A, top), the pattern of Rad53 phoshorylation by Western blot by using anti-Rad53 antibodies (A, bottom) and DNA content by fluorescence-activated cell sorting analysis (B). In A, genomic DNA was digested with XhoI and hybridized with a poly(GT) telomere-specific probe.