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. 2003 Aug;14(8):3144–3155. doi: 10.1091/mbc.E03-04-0212

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Copyright © 2003, The American Society for Cell Biology

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Figure 4. — Subcellular localization of endogenous LGN in cell lines. Confocal images of PC12 (A–C), WISH (d–F), and COS cells (G–L) stained for LGN (green) and DNA (blue) are shown. In all cell lines, LGN localizes to a perinuclear space during interphase (A, D, G, and J). Colocalization in interphase COS cells between LGN (J; green) and Vti1α Golgi marker (K; red) is shown in L (yellow). Note the cortical localization of LGN during mitosis is only observed in PC12 (B and C) and WISH (E and F) but not COS (H and I) cells. In metaphase (H) and anaphase (I) COS cells, LGN remains in the cytoplasm. Some cytoplasmic staining of LGN can also be seen in mitotic PC12 (B and C) and WISH (E and F) cells.