Figure 6.

Effects of cytoskeleton on LGN cortical localization. (A–L) Confocal images of cycling WISH cells stained with phalloidin (red), LGN (green), and DNA (blue); (A–C) a control interphase cell with LGN localizing at the perinuclear region (B, green) and actin microfilaments at the cell cortex (A, red); (C) a merged image of A and B; (D) the loss of cortical actin microfilaments upon latrunculin treatment, but the perinuclear LGN staining (E) remains unaffected in the drug-treated cell. (F) A merged image of D and E; (I) a merged image of G (phalloidin) and H (LGN) showing areas of overlap (yellow) between cortical actin and LGN in a control metaphase cell; note that some LGN staining in the cytoplasm is also visible at this stage. (J–L) A metaphase cell treated with latrunculin B; note the cytoplasmic staining of LGN (K) and the absence of phalloidin staining in the treated cell (J); (L) a merged image of J and K. (M and N) Metaphase WISH cells without (M) or with (N) colchicine treatment and stained for LGN (red), tubulin (green), and DNA (blue); note the absence of spindle staining in the treated cell, but no effect on LGN cortical staining (N).