Figure 9.

Effects of LGN on cell cycle progression. (A) Effect of ectopic expression of various mouse LGN constructs, FL-FLAG, N-FLAG, and C-FLAG on cell cycle in PC12 cells. Anti-BrdU and anti-FLAG antibodies were used for detection purposes. The bars represent percentage of BrdU-positive cells. The majority of FL-FLAG– or N-FLAG–transfected cells were BrdU-negative, whereas C-FLAG– or FLAG-vector–transfected cells were BrdU-positive, indicating that overexpression of either FL or N-FLAG but not C-FLAG results in cell cycle arrest. (B) Effect of LGN removal by morpholino treatment on cell cycle in PC12 cells. For control cells, the percentage of cells in G1 phase (M1) was ∼52%, S phase (M2) ∼11%, and G2 phase (M3) ∼37%. For morpholino-treated cells, the scores were ∼70% for M1, ∼10% for M2, and ∼21% for M3, indicating that the loss of LGN causes partial delay/disruption of the cell cycle progression at the G1/S phase boundary.