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. 2003 Aug;14(8):3169–3179. doi: 10.1091/mbc.E02-11-0715

Figure 6.

Figure 6.

(A1–A4) Typical metaphase-arrested spindle lacking astral microtubules after inactivation of bimE. A bimE7 strain (DBE4) was germinated at permissive temperature for a period to allow germination before shift to restrictive temperature of 42°C for 2 h. Cells were fixed and processed for immunofluorescence to visualize microtubules (A2) and stained with DAPI to reveal DNA (A3). A differential interference contrast image (A1) and a merge of the microtubule and DNA image (A4) are also shown. (B1–B3 and C1–C3 and D). Astral microtubules in a bimE7 + ΔtinA double mutant. A bimE7/ΔtinA strain (SO326) was geminated at permissive temperature for a period to allow germination before shift to restrictive temperature (42°C) for 2 h. Cells were fixed and processed to visualize microtubules (green) and stained with DAPI to reveal DNA (blue, but red in D to better distinguish individual nuclei in this cell). Arrows in B indicate a spindle-like structure stretching between DNA. Arrow in C indicates a bundle of astral microtubules. Arrows in D indicate astral microtubule bundles beginning to interact between nuclei. Bar, ∼5 μM.