Table 2.

Analysis of ciliary length and density for suppressor strain dilution series

Initial cell density (per ml)	Culture temp. (°C)	Cilia/μm cell periphery	Avg. length of measurable cilia μm ± SEM (n)	P value
1.5 × 105	30	0.25	2.9 ± 0.089 (236)	n/a
3.0 × 104	30	0.16	2.0 ± 0.112 (100)	1.0 × 10-5
3.0 × 103	30	0.15	1.4 ± 0.051 (256)	8.5 × 10-32
3.0 × 103	22	0.25	2.7 ± 0.076 (256)	0.018

n/a, not applicable.

Growing (shaken) IFT52Δsm cells were washed and resuspended in fresh MEPP at the indicated concentrations. After 21.5 h, cells were fixed and stained for immunofluorescence with anti-tubulin anti-bodies. Cilia were counted and measured on single confocal sections corresponding to the widest section through the macronucleus for a given z-series. Data are from a single representative experiment among many that were performed with very similar loss of motility in diluted cells. P values were generated using paired two-tailed t tests comparing means for all other dilutions to the mean for 1.5 × 10⁵ cells/ml.