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SWAP-70 does not localize on myosin II-containing actin fibers. B16F1 cells were transfected with GFP-SWAP-70 as described in MATERIALS AND METHODS and fixed for 3 min in 4% PFA, 0.1% Triton X-100 followed by fixation in 4% PFA for 30 min. Cells were labeled for myosin II by indirect immunofluorescence by using a secondary antibody coupled to Alexa 568. GFP-SWAP-70 (A), myosin II (B), and merge of the two stainings (C). Bar, 10 μm.
