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. 2003 Aug;14(8):3242–3253. doi: 10.1091/mbc.E03-01-0043

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Copyright © 2003, The American Society for Cell Biology

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Figure 7. — The PH-domain is necessary for proper localization of SWAP-70 to loose actin filament arrays, but it is not sufficient. B16F1 cells were transfected with GFP-SWAP-RR/EE (A and B) and PHGFP (E and F) or cotransfected with CFP-SWAP-70 (C) and YFP-SWAP-70R230C (D). GFP-SWAP-70-RR/EE– and PH-GFP–transfected cells were stained with Alexa 594-phalloidin (B and F). The cell shown in C and D was treated with AlF₄^– for 20 min. before fixation. Fluorescence images of representative cells are shown. In A–D, epifluorescence images are shown and in E, F a 0.4-μm confocal section. Bars, 10 μm.