Table 2.

Inhibition of various chain length BODIPY-LacCer analogs in rat fibroblasts

	% Inhibition
BODIPY-SL analogs	Nystatin	CPZ
C12 So, C5-BODIPY-LacCer	78.7 ± 11.2	9.8 ± 0.9
C16 So, C5-BODIPY-LacCer	80.8 ± 10.2	10.3 ± 1.1
C18 So, C5-BODIPY-LacCer	75.2 ± 8.9	9.3 ± 1.2
C20 So, C5-BODIPY-LacCer	75.9 ± 6.8	8.2 ± 1.2
C18 So, C3-BODIPY-LacCer	84.3 ± 9.4	5.4 ± 0.3

Cells were pretreated with or without the indicated inhibitor (see MATERIALS AND METHODS) and subsequently incubated with the indicated BODIPY-SL (see Figure 1 for structures) as described in Table 1. Values are mean ± SD of at least 10 cells in each of the three independent experiments. so, sphingosine.