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. 2003 Aug;14(8):3280–3291. doi: 10.1091/mbc.E03-03-0154

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Copyright © 2003, The American Society for Cell Biology

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Figure 2. — Role of Hsl1p kinase activity in phosphorylation and localization of Hsl1p, Hsl7p, and Swe1p. (A) Cells of strains DLY5000 (HSL1-myc HSL7-HA) and DLY5390 (hsl1^K110R-myc HSL7-HA) were grown to exponential phase, harvested, and lysed. Top, Western blot of myc-tagged Hsl1p or Hsl1p^K110R. Bottom, Western blot of hemagglutinin (HA)-tagged Hsl7p from HSL1 or hsl1^K110R strains. (B) Localization of Hsl1p, Hsl7p, and Swe1p in strains containing wild-type (top) or catalytically inactive (bottom) Hsl1p. Left, localization of Hsl1p-myc or Hsl1p^K110R-myc in strains DLY5000 and DLY5390. Middle, localization of Swe1p-myc in strains JMY1435 and DLY5019. Right, localization of Hsl7p-HA in strains DLY5794 and DLY5002.