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Timing of Hsl1p phosphorylation in bed1Δ mutants. Early G1 cells were isolated by centrifugal elutriation, inoculated into fresh medium, and fixed to examine Hsl1p recruitment to the septin cortex and bud formation. More than 200 cells were scored for each sample. The phosphorylation state of Hsl1p in the same samples was examined by Western blotting (bottom panels). (A) Strain DLY5000 (BED1 HSL1-myc). (B) Strain DLY5334 (bed1Δ HSL1-myc).
