Figure 2.

Schematic representation of protein interactions involving the Dam1 complex and the kinetochore proteins. (A) Model of Dam1 complex organization reflecting interactions identified in this study and in previously published two-hybrid assays (Ito et al., 2000, 2001; Uetz et al., 2000). (B) Interactions between Dam1p of the Dam1 complex and other kinetochore and spindle proteins. Kinetochore components that belong to discrete subcomplexes are grouped in solid boxes and the regulatory proteins are boxed in pink. The checkpoint proteins are grouped in the pink dashed box to show their related functions, not to imply physical association. Proteins interacting with Dam1p are color coded in this figure: red, interacting proteins that are identified in both two-hybrid and binding assays; green, interacting proteins that are identified only in binding assays; blue, interacting proteins that are identified only in two-hybrid screens; black, proteins that did NOT show any interactions with Dam1p; gray, proteins that were not tested in binding assays. MT, microtubules.