Figure 5.

UNC-76 and SYT do not colocalize in segmental nerves. (A) Shows the accumulation of UNC-76 in a nine-section optical projection (each section is 2 μm) of a segmental nerve from a Khc¹⁶/+; Df(3L)34ex5/+ individual that exhibits locomotion defects and SYT axon clogs (B). Note that UNC-76 accumulation is fairly uniform, whereas SYT is found in axon clogs (B). (C) Overlay of images in A and B shows that UNC-76 and SYT are largely nonoverlapping; upon viewing a single 2-μm optical section (D), it is apparent that UNC-76 is not detected in axon clogs. Bar, 25 μm.