Figure 6.

Addition of Fe-citrate to macrophages transduced with Rab5 WT or Ev-Neo had no effect on M. avium trafficking. BMMs infected with M. avium 101 were left untreated or retrovirally transduced with the Rab5 WT or Ev-Neo pLZRS constructs. The infected BMMs were incubated with Fe-citrate as described in Figure 5. The cells were fixed, permeabilized, and stained for either LAMP1 or TR. The number of mycobacterial phagosomes (n = 50 per experiment) that colocalize with LAMP1 or TR was determined and expressed as the mean ± SD for three separate experiments.