Figure 3.

CD1b tail targets the CD1b molecule to the limiting membrane of the lysosome. The localization of CD1b wt and CD1b tail chimera within LAMP1-positive compartments was determined in T2 cells transfected with the CD1b constructs (A). As a control, the localization of endogenous CD63 within these transfected cells was determined (B). Immunogold labeling with CD1b- or CD63-specific antibodies was performed on ultrathin cryosections, and the average number (and SE) of gold particles present on the limiting membrane and internal membranes was determined per lysosome.