Figure 7.

LPS stimulation matures DCs but did not raise cell surface levels of CD1 molecules. Monocyte-derived DCs cultured in GM-CSF and IL-4 were analyzed by flow cytometry to determine the expression of cell surface markers. Similar analyses were done on cells stimulated by addition of LPS for 48 h and control cells kept in GM-CSF, IL-4 medium for that period. Maturation marker CD83 is like MHC class II up-regulated in LPS-treated cells. Cell surface levels are not raised for CD1a, CD1b, and CD1c and lysosomal markers CD63 and LAMP1 on the LPS-treated cells. Data shown are representative for six separate experiments in which monocytes from different donors were used.