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Analysis of ppαF and preBiP translocation in the kar2 mutant strains. Each strain was grown at 26°C to midlog phase and total protein was labeled with 35S-methionine/cysteine for 5 min at either 26° (–) or 37°C (+). Cell extracts were prepared and ppαF and BiP were immunoprecipitated, resolved by SDS-PAGE, and visualized by phosphorimager analysis.
