Figure 2.

Concentration- and time-dependent HIF-1α accumulation. (A) HEK293 cells were stimulated with GSNO (0.1–2 mM) for 2 h or (B) with 1 mM GSNO for 15 min up to 4 h. Western analysis with an anti-HIF-1α mAb was performed as described in MATERIALS AND METHODS. Each experiment was performed at least three times and representative data are shown.