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In vitro effect of GSNO on PHD1 in hypoxia. GalDBD-HIF-1α549–582 was incubated with PHD1 in normoxia (lane 2) or in hypoxia in the absence (lane 3) or presence (lane 4) of 1 mM GSNO. The reaction was stopped by addition of 200 μM DFX. GalDBD-HIF-1α549–582 was precipitated with a Gal antibody, and 35S-pVHL was added. Detection of pVHL indicates enzymatic hydroxylation of the HIF-1α-ODD.
