Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
C-terminal HIF-1α transactivation after GSNO-treatment. HEK293 cells, 2 × 105, were cotransfected with 0.5 μg pGal-HIF-1α727–826 and 1 μg pGal-Luc plasmids and stimulated for 16 h with 1 mM GSNO, exposed to 1% hypoxia, or left untreated (control). After cell lysis, luciferase activity was measured and normalized compared with controls. Each experiment was performed at least three times and representative data are shown. Data are the mean ± SD (n = 3).
