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Slow-folding RFP predominantly labels one of the two SPBs of an Spc110p-RFP cell. (A) Spc110p was tagged at the C terminus with RFP (TYY115-2D). Cells from an exponentially growing culture were fixed and imaged as described in MATERIALS AND METHODS. Bar, 5 μm. (B) The cellular position of the more brightly labeled SPB with respect to the bud neck was recorded in >200 cells.
