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FRAP analysis of Spc110p-GFP diploid cells (TYY113) in G2 with short spindles. Little Spc110p is exchanged in G2. One SPB (arrowhead) in a G2 cell was photobleached, N = 22. (A) Image of a representative cell before photobleaching. (B) The same cell as in A immediately after photobleaching. (C) A final z-series was acquired in the fluorescence channel after ∼30 min. Time is in minutes. Bar, 2 μm. (D) Histogram plot of the percentage of recovery.
