Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1981 Aug;20(2):252–257. doi: 10.1128/aac.20.2.252

Determination of moxalactam in human body fluids by liquid chromatographic and microbiological methods.

D J Miner, D L Coleman, A M Shepherd, T C Hardin
PMCID: PMC181673  PMID: 6456691

Abstract

High-performance liquid chromatographic methods for determination of the isomers of moxalactam in plasma and urine have been developed. Conventional reverse-phase chromatography was used for plasma assays, and an ion-pairing reagent was included for urine assays. Detection limits were 1.5 micrograms/ml of plasma and 7.5 micrograms/ml of urine. The high-performance liquid chromatographic assays were extensively compared with a microbiological assay (detection limit, 1 microgram/ml), using samples from human volunteers to whom moxalactam had been administered as well as plasma and urine from untreated humans, to which moxalactam was added. The correlations between the assays were quite good, but the precision and accuracy of the high-performance liquid chromatographic methods were superior. Both types of assays were used in a study of the stability of moxalactam-containing samples at various temperatures.

Full text

PDF
257

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barza M., Tally F. P., Jacobus N. V., Gorbach S. L. In vitro activity of LY127935. Antimicrob Agents Chemother. 1979 Sep;16(3):287–292. doi: 10.1128/aac.16.3.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Diasio R. B., Wilburn M. E., Shadomy S., Espinel-Ingroff A. Rapid determination of serum 5-fluorocytosine levels by high-performance liquid chromatography. Antimicrob Agents Chemother. 1978 Mar;13(3):500–504. doi: 10.1128/aac.13.3.500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gibbs R. S., Blanco J. D., Castaneda Y. S., St Clair P. J. Therapy of obstetrical infections with moxalactam. Antimicrob Agents Chemother. 1980 Jun;17(6):1004–1007. doi: 10.1128/aac.17.6.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Maitra S. K., Yoshikawa T. T., Hansen J. L., Nilsson-Ehle I., Palin W. J., Schotz M. C., Guze L. B. Serum gentamicin assay by high-performance liquid chromatography. Clin Chem. 1977 Dec;23(12):2275–2278. [PubMed] [Google Scholar]
  5. Nakagawa T., Haginaka J., Yamaoka K., Uno T. High speed liquid chromatographic determination of cephalexin in human plasma and urine. J Antibiot (Tokyo) 1978 Aug;31(8):769–775. doi: 10.7164/antibiotics.31.769. [DOI] [PubMed] [Google Scholar]
  6. Neu H. C., Aswapokee N., Fu K. P., Aswapokee P. Antibacterial activity of a new 1-oxa cephalosporin compared with that of other beta-lactam compounds. Antimicrob Agents Chemother. 1979 Aug;16(2):141–149. doi: 10.1128/aac.16.2.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nilsson-Ehle I., Yoshikawa T. T., Schotz M. C., Guze L. B. Quantitation of antibiotics by high-pressure liquid chromatography: cephalothin. Antimicrob Agents Chemother. 1978 Feb;13(2):221–227. doi: 10.1128/aac.13.2.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Parsons J. N., Romano J. M., Levison M. E. Pharmacology of a new 1-oxa-beta-lactam (LY127935) in normal volunteers. Antimicrob Agents Chemother. 1980 Feb;17(2):226–228. doi: 10.1128/aac.17.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wold J. S., Turnipseed S. A. Determination of cephaloridine in serum and tissue by high-performance liquid chromatography. J Chromatogr. 1977 Jun 1;136(1):170–173. doi: 10.1016/s0021-9673(00)83008-3. [DOI] [PubMed] [Google Scholar]
  10. Wold J. S., Turnipseed S. A. The simultaneous quantitative determination of cephalothin and cefazolin in serum by high pressure liquid chromatography. Clin Chim Acta. 1977 Jul 15;78(2):203–207. doi: 10.1016/0009-8981(77)90307-2. [DOI] [PubMed] [Google Scholar]
  11. Yamada H., Ichihashi T., Hirano K., Kinoshita H. Plasma protein binding and urinary excretion of R- and S-epimers of an arylmalonylamino 1-oxacephem. I: In humans. J Pharm Sci. 1981 Jan;70(1):112–113. doi: 10.1002/jps.2600700130. [DOI] [PubMed] [Google Scholar]
  12. Yoshida T., Matsuura S., Mayama M., Kameda Y., Kuwahara S. Moxalactam (6059-S), a novel 1-oxa-beta-lactam with an expanded antibacterial spectrum: laboratory evaluation. Antimicrob Agents Chemother. 1980 Mar;17(3):302–312. doi: 10.1128/aac.17.3.302. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES