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Two populations of in vitro-uncoated clathrin-coated vesicles. Clathrin-coated vesicles were uncoated in vitro and repurified in a 10–50% sucrose equilibrium density gradient. Aminopeptidase-containing and non-aminopeptidase-containing fractions were combined separately and centrifuged in a continuous 10–30% sucrose gradient. Fractions were analyzed for total protein (•) and aminopeptidase activity (◊) (A) and individual synaptic vesicle proteins by Western blotting (B).
