Figure 2.

Activation of a p53-responsive reporter gene by HDMAS5. JAR cells stably transfected with the p53-inducible BP100-luciferase plasmid (JAR-BP100-luc) were treated with 200 nM oligonucleotide for 24 h. Luciferase activities in the treated cells were determined and shown as luciferase activity/unit protein. HDMAS5 activated the p53-responsive reporter expression by 7-fold. JAR cells stably transfected with a luciferase reporter driven by the tk promoter (JAR-TK-luc) and H1299 cells (p53-null) stably transfected with BP100-luciferase (H1299-BP100-luc) were tested similarly. HDMAS5 did not activate the reporters in these controls. The results are the average of at least four experiments for each data point.