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The segment of DNA (Lower) crossed into the chromosome of a cheA deletion strain (Upper) to yield strain HCB900, drawn to the same scale. The shaded regions are regions of homology used in the cross-in. cheY was replaced by cheY13DK106YW and most of cheZ was deleted. Base pairs are numbered and fragments are labeled as in Table 2. Arrows, transcription units; arrowheads, hybridization sites for primers; open boxes and lines, genomic DNA; solid boxes, lines, and circle, plasmid DNA.
