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. 2007 Feb 15;26(5):1434–1443. doi: 10.1038/sj.emboj.7601575

Figure 1.

Figure 1

ABA levels increase during a compatible interaction in an inoculum concentration-dependent manner. (A) Time course of changes in ABA levels in Col-5 leaves after mock infiltration (MgCl2, 10 mM) or challenge with DC3000 or DC3000 hrpA−. (B) ABA levels are dependent on DC3000 concentration. ABA levels in leaves challenged with DC3000 hrpA− or increasing concentrations of DC3000 were measured 18 hpi. (C) Relative in planta levels of ABA, JA and SA during compatible (DC3000) and basal defence (DC3000hrpA−) responses. Hormones were measured in both non-inoculated and challenged (0.5 × 108 CFU/ml) Col-5 leaves at 18 hpi. (D) 9-cis-Epoxycarotenoid-dioxygenase3 (NCED3) is induced by T3Es concomitant with elevated foliar ABA. Time course of NCED3 steady-state mRNA levels following DC3000 and DC3000hrpA− challenge determined, in independent experiments, by RNA blot (upper panel) or RT–PCR. Bacterial titres and RT–PCR values (copies of target transcript/copy actin 2) are means of triplicate samples and error bars represent 1 s.d. All experiments were repeated at least twice with similar results.