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. 2007 Feb 22;26(5):1257–1267. doi: 10.1038/sj.emboj.7601596

Figure 2.

Figure 2

Receptors required for CCN1/TNFα-induced apoptosis. Primary HSFs were subjected to various treatments as described below, and then treated with soluble CCN1, TNFα, or both for 4.5 h before scoring for apoptosis. (A) HSFs were pre-incubated with 100 μg/ml mAbs that neutralize TNFRI, TNFRII, or with anti-syndecan-4 polyclonal antibodies (100 μg/ml), or normal rabbit IgG for 30 min before treatment with CCN1 and TNFα. (B) Cells were incubated with various inhibitory mAbs (50 μg/ml) before treatment with CCN1 and/or TNFα. Antibodies used included GoH3 (anti-integrin α6), P5D2 (anti-β1), LM609 (anti-αvβ3), P1F6 (anti-αvβ5), and normal mouse IgG. (C) HSFs were treated with CCN1 or the mutants DM, TM, or D125A (4 μg/ml each), or a combination of DM and D125A, either with or without TNFα.