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Skeletal muscle extract proteins were immunoblotted for phospholamban expression (PLN; top panel) and phospho-Thr17 PLN (middle panel). The bottom panel is signal intensity for phospho-Thr17 PLN relative to PLN expression. Representative immunoblots are shown. Data are mean ± s.e.m., n = 8. †P < 0.01 versus time 0; *P < 0.05 versus time 0.
