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Skeletal muscle membrane proteins were extracted and subject to phosphorylation assays as described in Methods. A, expression of PLN, CaMKII and SERCA2 in different fractions. B, PLN and CaMKII phospho-Thr level and expression after different incubation conditions. Representative immunoblots are shown. Data are mean ± s.e.m., n = 3. †P < 0.01 versus −/−; *P < 0.001 versus +/+.
