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. 2006 Apr 27;574(Pt 1):41–53. doi: 10.1113/jphysiol.2006.108506

Figure 3. Effect of dietary PUFAs on L-PK and ChREBP gene expression and ChREBP localization in livers of AMPKα1α2LS−/− mice.

Figure 3

A, quantitative RT-PCR analysis of L-PK and ChREBP gene expression from livers of 24 h-fasted mice (F) and mice re-fed for 18 h with a high carbohydrate diet (HCHO) supplemented or not with PUFAs (PUFA) performed in control (filled bars) and AMPKα1α2LS−/− (open bars) mice. Results are means ± s.e.m.; n = 3/group. *Significantly different from mice re-fed with HCHO diet for 18 h (P < 0.005). B, cytosolic and nuclear ChREBP content from livers of 24 h-fasted control and AMPKα1α2LS−/− mice re-fed for 18 h upon HCHO diet supplemented or not with PUFAs. Expression of AMPKα catalytic subunits has been measured by using anti-pan-AMPKα antibodies. β-Actin protein levels are presented as loading control. A representative Western blot is shown; n = 3/group.