Table 1.
Anti-tumour effects of E7 DNA vaccine cassettes
| Days post tumour challenge1 | ||||||
|---|---|---|---|---|---|---|
| Plasmids | 8 | 15 | 20 | 34 | 42 | 50 |
| Neg. control (pLacZ) | 7 | 8 | 8 | 9 | 9 | – |
| pcDNA3-E7 | 6 | 8 | 8 | 9 | 9 | – |
| pcDNA3-Sig/E7 | 0 | 0 | 0 | 0 | 02 | 0 |
| pcDNA3-Sig/E7/TMR | 2 | 2 | 3 | 3 | 32 | 3 |
| pcDNA3-Sig/E7/LAMP | 0 | 0 | 0 | 0 | 02 | 0 |
Each group of mice (n = 10) was immunized i.m. with 50 μg of E7 plasmid DNA cassettes at 0, 2 and 6 weeks and then challenged s.c. with 1 × 104 TC-1 tumour cells per mouse at 8 weeks. Mice were counted for tumour formation over time. This was repeated with similar results.
1
The values represent the number of animals with tumour; the number of animals challenged each time being 10.
2
Statistically significant using Chi-square test at P < 0·05 compared to negative controls.