Figure 2.

Blood CD4⁺ T cells are retained to a lesser degree than other subsets during DTH-induced shut-down. The number of all measured lymphocyte subsets decreases during the period of lymph node shut-down. (a) CD8 (▪), γδ (▴) and CD21 (•) percentages also decrease during lymph node shut-down but the percentage of CD4 (♦) increases (b). To determine which of the pools of lymphocytes was responsible, three-colour FACs analysis was performed. The CFSE-labelled blood pool of lymphocytes (c) shows an increase in the CD4 subset. The recirculating lymph pool of lymphocytes labelled with DiI-DS also shows an increase in the percentage of CD4 cells (d), but not as large as the blood pool of lymphocytes. Ratios are calculated as in Fig. 1, and are the means of three animals. Arrow indicates time of PPD injection (results presented as mean ± SEM).