Figure 3.

Efferent lymph plasma collected during shut-down significantly increases lymphocytes binding to lymphatic endothelial cells in vitro. Lymph plasma was collected from the efferent lymph draining a DTH-stimulated lymph node, and cells removed by centrifugation. Individual samples were incubated with confluent cultures of lymphatic endothelium in the well-established binding assay. Numbered samples in the upper panel correspond to the lymph sample number in the lower panel. Samples obtained during lymph node shut-down significantly increased the binding of lymphocytes to lymphatic endothelium relative to other lymph samples (*P < 0·05, anova).