Table 1.

Differential binding of blood versus lymph CD4 cells to lymphatic endothelium¹

Experimental conditions	Blood CD4 cells	Lymph CD4 cells
EC† + media	6·15 ± 0·49*	2·85 ± 0·26
EC + 1 ng IFN-γ	8·96 ± 1·14	6·01 ± 0·49‡
EC + 10 ng IFN-γ	11·58 ± 1·49‡	7·00 ± 0·71‡
EC + 100 ng IFN-γ	12·08 ± 1·16‡	6·24 ± 0·77‡
EC + 100 ng TNF	10·2 ± 2·99	4·04 ± 0·49

¹PBL and ELL were separated by standard panning methods and radiolabelled. Lymphatic endothelium was stimulated with cytokines for 17 hr, washed and radiolabelled lymphocytes added. After 1 hr non-adherent lymphocytes were removed by washing and the remaining radioactivity determined.

^*Average ± SEM of the percentage bound of the added lymphocytes in four experiments.

^†EC = lymphatic endothelial cell.

^‡Statistically significant (P < 0·05) compared to media alone.