Table 2.
Analysis of distribution of somatic mutations in different antibodies
| Clone | Chain | Germline gene | Expected R/S in FR† | Observed R/S in FR | P for FR | Expected R/S in CDR† | Observed R/S in CDR | P for CDR |
|---|---|---|---|---|---|---|---|---|
| 5S | VL | IGKV1-117*02 | 6·14/2·07 | 3/1 | 0·031 | 2·16/0·62 | 4/3 | 0·093 |
| VH | J558·42 | 11·01/3·67 | 9/3 | 0·180 | 3·48/0·83 | 4/3 | 0·365 | |
| 5C3 | VL | IGKV3-1*01 | 5·16/1·63 | 4/4 | 0·228 | 1·72/0·48 | 1/0 | 0·694 |
| VH | IGHV2S4*01 | 6·42/2·12 | 7/3 | 0·626 | 1·89/0·57 | 1/0 | 0·730 | |
| H67 | VL | IGKV3-2*01 | 1·16/0·36 | 1/0 | 0·418* | 0·38/0·1 | 1/0 | 0·191* |
| VH | IGHV1S8*01 | 7·65/2·43 | 2/7 | 0·001 | 2·41/0·5 | 4/0 | 0·143 | |
| C6-9-1 | VL | IGKV8-30*01 | 1·12/0·37 | 1/0 | < 0·001* | 0·41/0·10 | 1/0 | 0·205* |
| VH | IGHV1S133*01 | 8·13/2·73 | 5/7 | 0·132 | 2·49/0·65 | 2/0 | 0·838 | |
| T6J | VL | IGKV3-15*01 | 1·79/0·59 | 0/1 | 0·033* | 0·46/0·15 | 1/1 | 0·229* |
| VH | IGHV3-30*04 | 9·18/3·23 | 4/2 | 0·005 | 2·86/0·74 | 7/3 | 0·009 | |
| CL4MAb | VL | IGKV3-15*01 | 2·39/0·79 | 0/2 | 0·013* | 0·62/0·2 | 2/0 | 0·064* |
| VH | IGHV3-30*04 | 12·05/4·23 | 7/2 | 0·015 | 3·75/0·97 | 10/2 | 0·001 | |
| 23HN | VL | IGKV3-20*01 | 4·72/1·57 | 4/2 | 0·309 | 1·32/0·39 | 1/1 | 0·576 |
| VH | IGHV3-33*01 | 12·65/4·44 | 5/8 | 0·001 | 3·97/0·93 | 7/2 | 0·062 | |
| SOJA | VL | IGKV3-11*01 | 4·77/1·60 | 2/2 | 0·031 | 1·27/0·36 | 4/0 | 0·015 |
| VH | IGHV3-23*01 | 4·03/1·4 | 0/0 | 0·001 | 1·23/0·35 | 6/1 | < 0·001 | |
| SOJB | VL | IGLV3-21*03 | 8·13/2·98 | 6/2 | 0·133 | 2·27/0·63 | 5/1 | 0·039 |
| VH | IGHV2-5*10 | 11·96/4·16 | 9/9 | 0·068 | 3·75/1·31 | 2/2 | 0·851 | |
| CB STL-1 | VL | IGLV3-25*03 | 12·59/4·52 | 10/6 | 0·138 | 3·78/1·12 | 6/0 | 0·117 |
| VH | IGHV1-46*03 | 19·72/6·64 | 13/8 | 0·011 | 6·22/1·41 | 9/4 | 0·117 | |
| 1F6 | VL | IGKV1-110*01 | 1·13/0·38 | 1/0 | 0·435* | 0·38/0·11 | 0/1 | 0·674* |
| VH | IGHV1S137*01 | 4·69/1·49 | 2/5 | 0·034 | 1·48/0·33 | 1/0 | 0·630 | |
| 78·2 | VL | IGKV5-43*01 | 0/0 | 0/0 | – | 0/0 | 0/0 | – |
| VH | IGHV8S7*01 | 6·16/2·17 | 1/2 | 0·009 | 2·16/0·50 | 6/2 | 0·006 | |
| MAR | VL | 2b2 (lambda II) | 10·25/3·54 | 9/5 | 0·278 | 3·14/1·06 | 4/0 | 0·289 |
| PAP | VL | lambda III.1 | 11·77/4·09 | 7/4 | 0·018 | 3·21/0·93 | 9/0 | 0·001 |
| U86366 | VH | DP 65 | 9·63/3·38 | 8/7 | 0·228 | 4·81/1·17 | 2/2 | 0·933 |
| AF004935 | VH | COS 16 | 9·15/2·79 | 5/3 | 0·024 | 3·94/1·11 | 9/0 | 0·005 |
†
Calculated using Chang and Casali's method. P = 0·05 are shown in bold type.
*
Cases where the total number of mutations is very low (≤ 4) and a multinomial distribution model (with a cut-off value of P = 0·05) cannot be used. Germline sequences retrieved from the imgt database are shown in imgt format (i.e. gene name * allele number).