. 2005 Oct;116(2):172–183. doi: 10.1111/j.1365-2567.2005.02208.x

Table 3.

Analysis of distribution of somatic mutations in different antibodies using the modified method

		R/S in FR				R/S in CDR

Clone	Chain	Expected	Observed	P for FR	Selection against R in FR	Expected	Observed	P for CDR	Selection for R in CDR
5S	V_L	5·71/1·93	3/1	0·058	No	2·61/0·75	4/3	0·173	No
	V_H	10·44/3·54	9/3	0·257	No	3·89/1·13	4/3	0·457	No
5C3	V_L	4·89/1·56	4/4	0·284	No	1·99/0·56	1/0	0·759	No
	V_H	6·04/1·99	7/3	0·708	No	2·28/0·69	1/0	0·810	No
H67	V_L	1·1/0·34	1/0	0·451^*	NA	0·45/0·11	1/0	0·224^*	NA
	V_H	7·31/2·32	2/7	0·002	Yes	2·79/0·58	4/0	0·211	No
C6-9-1	V_L	1·08/0·36	1/0	0·462^*	NA	0·45/0·11	1/0	0·226^*	NA
	V_H	7·67/2·57	5/7	0·083	No	2·98/0·78	2/0	0·715	No
T6J	V_L	1·77/0·55	0/1	0·034^*	NA	0·54/0·14	1/1	0·268^*	NA
	V_H	8·68/2·75	4/2	0·012	Yes	3·78/0·79	7/3	0·04	Yes
CL4MAb	V_L	2·36/0·73	0/2	0·014^*	NA	0·72/0·19	2/0	0·086^*	NA
	V_H	11·39/3·61	7/2	0·03	Yes	4·96/1·04	10/2	0·009	Yes
23HN	V_L	4·42/1·48	4/2	0·386	No	1·62/0·48	1/1	0·671	No
	V_H	11·76/4·14	5/8	0·002	Yes	4·94/1·26	7/2	0·153	No
SOJA	V_L	4·52/1·57	2/2	0·045	Yes	1·49/0·42	4/0	0·026	Yes
	V_H	3·74/1·3	0/0	0·002	Yes	1·53/0·43	6/1	< 0·001	Yes
SOJB	V_L	8·04/2·94	6/2	0·144	No	2·36/0·65	5/1	0·046	Yes
	V_H	12·03/4·18	9/9	0·102	No	4·45/1·34	2/2	0·902	No
CB STL-1	V_L	12·5/4·48	10/6	0·147	No	3·88/4·48	6/0	0·127	No
	V_H	18·04/6·08	13/8	0·044	Yes	8·05/1·83	9/4	0·343	No
1F6	V_L	1·07/0·35	1/0	0·467^*	NA	0·45/0·13	0/1	0·700^*	NA
	V_H	4·39/1·4	2/5	0·054	No	1·81/0·4	1/0	0·719	No
78·2	V_L	0/0	0/0	–	NA	0/0	0/0	–	NA
	V_H	5·91/2·08	1/2	0·002	Yes	2·44/0·57	6/2	0·012	Yes
MAR	V_L	9·95/3·43	9/5	0·329	No	3·45/1·17	4/0	0·358	No
PAP	V_L	11·47/3·99	7/4	0·025	Yes	3·52/1·02	9/0	0·003	Yes
U86366	V_H	9·21/3·19	8/7	0·296	No	5·31/1·29	2/2	0·958	No
AF004935	V_H	8·8/2·65	5/3	0·036	Yes	4·33/1·22	9/0	0·009	Yes

P-values = 0·05 are shown in bold type.

Cases where the total number of mutations is very low (≤4). Inferences drawn from the statistics are given as yes/no. NA = not applicable.