Figure 2.

Differential effects of CpG-DNA in cross-tolerance induction in vitro. (a) Spleen cells or (b) thioglycollate-induced peritoneal macrophages from Balb/c mice were stimulated with either 100 nm CpG-oligodesoxynucleotides (ODN) (C) or 3 µg/ml lipoteichoic acid (LTA) (L) for 18 or 28 hr, respectively. Subsequently, cells were washed twice in phosphate-buffered saline and once in medium and then were restimulated with either CpG-ODN or LTA as above. Eight hours later, tumour necrosis factor (TNF)-α and interleukin (IL)-12p40 concentrations were measured in the supernatant. Mean values of duplicates + standard deviation for one of two experiments are shown.