Figure 3.

Effects of protein kinase C (PKC) inhibitors on eosinophil adhesion. Purified human eosinophils (5 × 10⁴ cells/well) were stimulated with platelet-activating factor (PAF) (1 µm) or complement component 5a (C5a) (100 ng/ml) in the presence or absence of PKC inhibitor in a human serum albumin-coated plate at 37°. After 1 hr, percentages of eosinophils adherent to the wells of the plate were determined. Data are expressed as mean ± standard error of the mean (SEM) for four independent experiments. Percentage adhesion of control cells in the absence of drugs was as follows: medium alone, 4·3 ± 0·9%; PAF, 14·1 ± 1·7%; and C5a, 15·9 ± 1·9% (mean ± SEM; n = 4). Total cellular EPX content was 2817·7 ± 235·8 ng/10⁶ cells (mean ± SEM; n = 4). *P < 0·05; significant differences compared with cells stimulated with PAF or C5a in the absence of inhibitor.