Table 1.
IC50 values of protein kinase C (PKC) inhibitors for superoxide anion generation induced by platelet-activating factor (PAF) or complement 5a (C5a)
| [IC50 (µm)] | ||
|---|---|---|
| PAF | C5a | |
| PKC ζ inhibitor | 0·8 | 0·9 |
| BisI | 1·5 | 0·1 |
| Peptide 20-28 | 5·1 | 7·5 |
| Rottlerin | 0·5 | 0·2 |
Purified human eosinophils in human serum albumin-coated wells (5 ×104 cells/well) were stimulated with PAF (1 µm) or C5a (100 ng/ml) after preincubation with each PKC inhibitor for 15 min. IC50 values of PKC inhibitors (µm) were calculated from the values of superoxide anion generation for 30 min after stimulation by PAF or C5a.
BisI, bisindlolylmaleimide I.