Table 2.

Effects of protein kinase C (PKC) inhibitors on CD11b expression on eosinophils

	Mean fluorescence intensity (MFI)
	PAF	C5a
PKC ζ inhibitor	91·6 ± 3·1	103·3 ± 4·4
BisI	145·9 ± 7·4**	125·5 ± 12·3
Peptide 20-28	101·9 ± 9·6	118·0 ± 7·2
Rottlerin	88·1 ± 6·5	127·8 ± 16·4

Purified eosinophils (2·5 ×10⁵ cells/sample) were stimulated with or without 1 µm platelet-activating factor (PAF) or 100 ng/ml complement 5a (C5a) after pretreatment with PKC inhibitor (3 µm PKC ζinhibitor, 1 µmBisI, 10 µm peptide 20-28 or 10 µm rottlerin) or medium alone for 15 min at the concentrations indicated. Expression of CD11b was determined by flow cytometric analysis as mean fluorescence intensity (MFI). The MFI in the absence of inhibitors was as follows: medium alone, 14·3 ± 1·1; PAF, 22·8 ± 1·7; and C5a, 24·2 ± 0·6 (mean ± standard error of the mean (SEM); n = 5–9).

^**P < 0·01; significant differences compared with the cells stimulated with PAF or C5a in the absence of inhibitors.

BisI, bisindlolylmaleimide I.