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. 2005 Oct;116(2):223–232. doi: 10.1111/j.1365-2567.2005.02213.x

Figure 5.

Figure 5

Calcium mobilization by CD20 hypercrosslinking is cholesterol-dependent. Ramos B cells were loaded with fluo-3 and then incubated in the presence or absence of MβCD; some MβCD treated samples were further incubated with MβCD/cholesterol. Cells were then incubated with rituximab or 2H7. After establishing the baseline, cells were stimulated with either 5 µg GAH or 50 µg RAM (top and middle panels). Bottom panel, cells were stimulated with 10 µg F(ab′)2 anti-human IgM. Data shown are representative of at least three independent experiments.