Figure 6.

Apoptosis induced by CD20 crosslinking is cholesterol-dependent. Ramos B cells were incubated in the absence or presence of MβCD; some MβCD treated samples were further treated with MβCD/cholesterol. Cells were washed and incubated in serum-free medium with the antibodies indicated. After 4 hr FBS was added to each well and incubation continued for an additional 11 hr. Cells were stimulated with (a) 2H7 or (b) rituximab and stained with AnnV and PI. Data shown are the average and standard error of at least three independent experiments. Significant differences between mean results are indicated (**P < 0·0049; *P < 0·0241).