Fig. 2.

Expression array analysis of IFN-γ-treated human WISH cells. Human WISH cells were treated with either medium, 100 units/ml IFN-γ, 10 μg/ml IL-1Ra, or their combination. Total RNA was isolated at 17 h and subjected to expression-array analysis (Affymetrix U133A chip). Using Excel software, the Affymetrix data files were sorted by the algorithm described in Materials and Methods. Shown is the fold induction of genes in cells treated with IFN-γ for 17 h in the absence (hatched bars) or presence (open bars) of IL-1Ra. The values in parentheses are the average signal of two replicate assays. All signals and fold inductions were considered significant by the Affymetrix software (P < 0.05). Gene induction and array analyses were performed twice with very similar results.