Skip to main content
. Author manuscript; available in PMC: 2007 Oct 1.
Published in final edited form as: Alcohol. 2006 Oct;40(2):111–118. doi: 10.1016/j.alcohol.2006.11.001

Fig. 1. In Vitro EtOH Exposure Paradigm.

Fig. 1

(A) Scheme illustrating the procedure used to expose neonatal (P4–P6) hippocampal slices to 50 mM EtOH for 3–4 hrs. Sister slices were segregated into a chamber that contained continuously oxygenated ACSF solution with or without EtOH. Left and right sister slices were randomly assigned to each chamber. All slices were allowed to equilibrate for a period greater than 80 min prior to the start of the experiment. (B) EtOH concentrations in the ACSF do not significantly change as a function of time. Numbers in each bar represent the number of experiments.