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One-step virus growth in the presence and absence of rifampicin. BS-C-1 cells were infected with 3 PFU per cell of wild type VACV WR or mutants designated by the codon substitution in the D13L gene. After the 1 h adsorption, the cells were incubated for 0, 8, 21 or 44 h in the absence (uninterrupted line) or presence (dashed line) of 100 μg of rifampicin per ml. Virus titers were determined by plaque assay in the absence of rifampicin.
